If you're interested in exploring your ancient or modern ancestry using G25 coordinates (also called Global25), this guide will show you exactly how to use them with online calculators even if you don't yet have your real coordinates.
You can either:
You may also want to check:
What Is Raw DNA Data, How to Download It, and Where to Upload It for More Insights
Step 1: Choose a G25 Admixture Calculator
Visit one of the compatible G25 calculators : https://www.exploreyourdna.com/calculators.aspx
For this tutorial, we’ll use the following one which is a calculator that contains modern references :
Modern World Regions (Mythbuster General)
This calculator works with both real and simulated G25 coordinates.
Step 2: Prepare Your Coordinates
If you don’t have your real G25 yet, you can paste this sample to test:
Simulated Coordinates (example)
sample_simulated_g25_scaled,0.122903,0.112141,0.052407,0.026480,0.027892,0.006254,-0.015992,-0.018563,0.007526,0.010034,-0.002762,0.003465,-0.008828,-0.004363,0.004469,0.001165,-0.001619,0.001108,0.003116,-0.000511,0.000786,0.002215,0.000254,0.006655,-0.000435
You can paste this line just below the “Target” tab in the calculator input box.
Step 3: Choose "No" for Aggregate Mode
On most calculators, you’ll be asked whether to use "aggregate mode".
For clearer breakdowns with specific populations, select "NO".
Example Result – Aggregate Mode Enabled
Target: sample_simulated_g25_scaled
Distance: 0.2506% / 0.00250562
29.2 Northwest_European:Norwegian
10.4 Northeast_Italian:Italian_Northeast
9.8 Northwest_European:English
8.6 Northwest_Italian:Italian_Lombardy
7.2 Greek:Greek_Central_Macedonia
7.2 Southeast_European:Serbian
6.2 Northwest_European:Scottish
5.2 Sardinian:Sardinian
3.8 Iberian:Spanish_Extremadura
3.6 South_Andean:Aymara
3.4 Northwest_Italian:Italian_Piedmont
2.8 North_Andean:Quechua
1.4 Eastcentral_European:Czech
1.2 East_European:Ukrainian_Rivne
Example Result – ADC 0.25 Mode Enabled
When comparing your genetic coordinates to those of known populations, the calculator tries to find the best mix of populations that minimizes the genetic distance. But sometimes, if left uncorrected, the model might pick many similar populations to reduce the mathematical error, even if it's not biologically meaningful.
To avoid overfitting, ADC adds a penalty to the use of too many reference populations. The lower the ADC value, the stronger the penalty, encouraging the model to use fewer, more distinct populations.
| ADC Value |
Effect on Results |
Use Case |
| 0 |
No penalty, many populations |
Mathematical best fit |
| 0.25 |
Light penalty, simplified breakdown |
Most common for public calculators |
| 0.5+ |
Strong penalty, few populations |
Biogeographic modeling or summaries |
Target: sample_simulated_g25_scaled
Distance: 0.4090% / 0.00408967 | ADC: 0.25x RC
32.2 West_European:French_Occitanie
29.2 Southeast_European:Romanian
21.8 Northwest_European:Scottish
10.0 West_European:French_Paris
6.6 North_Andean:Quechua
0.2 Southwest_Canadian_Indigenous:Cree
Interpretation of the Results
This individual’s ancestry is predominantly European, with additional trace ancestry from the Americas:
- Northwest European: Includes Norwegian, English, and Scottish components (~45%)
- Southeast and Southern European: Includes Italian, Greek, Serbian, and Iberian contributions (~40%)
- Amerindian (Native South American): Includes Aymara and Quechua (~7%)
- Other minor components: Small percentages from Eastern European and Sardinian groups
These simulated results can give a basic picture of possible ancestries. For more precise interpretation, use your real DNA coordinates from G25 Requests.
Summary
- Using real G25 coordinates will yield the most accurate results. You can order them from G25 Requests.
- For testing or educational purposes, you can use simulated coordinates.
- The results will show a detailed breakdown of your ancestry with percentages and population labels.
- Be sure to select the correct mode ("NO" for most clear breakdowns).
For more tips and guides, visit ExploreYourDNA.com.
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